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goat anti rabbit biotinylated secondary antibody  (Jackson Immuno)


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    Structured Review

    Jackson Immuno goat anti rabbit biotinylated secondary antibody
    Goat Anti Rabbit Biotinylated Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+goat+anti+rabbit+antibody/Biotin-SP+(long+spacer)+AffiniPure+F(ab')%E2%82%82+Fragment+Goat+Anti-Rabbit+IgG%2C+Fc+fragment+specific/pmc12996694-111-14-22
    Average 93 stars, based on 34 article reviews
    goat anti rabbit biotinylated secondary antibody - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Anorectic and anxiogenic actions of cocaine– and amphetamine-regulated transcript in the lateral septum
    Article Snippet: The sections were then blocked in 3% normal donkey serum (NDS; 017-000-121, Jackson ImmunoResearch Laboratories, West Grove, PA; RRID: AB_2337258) for 2 h and immediately incubated with a rabbit anti-c-Fos antibody (1:2,000; ab190289, abcam, Cambridge, UK; RRID:AB_2737414) at RT overnight (16 h). .. After six 5-min washes in PBS (RT), the slices were incubated at RT for 1 h with a biotinylated goat anti-rabbit antibody (1:500; 111-065-144, Jackson ImmunoResearch Laboratories; RRID:AB_2337965) and then washed again three times in PBS for 10 min each. .. The sections were then treated with avidin biotin horseradish peroxidase (1:500; PK-6100; Vector Laboratories, Newark, CA) in PBS for 1 h at RT.

    Article Title: Inhibitory actions of melanin‐concentrating hormone in the lateral septum
    Article Snippet: .. The following day, the tissue was washed six times in PBS (5 min each) then incubated with a biotinylated goat anti-rabbit antibody (1:500; Jackson ImmunoResearch Laboratories; RRID: AB_2337965) prepared in NDS for 1 h (RT). ..

    Article Title: Tree shrew as a new animal model for musculoskeletal disorders and aging
    Article Snippet: Next, after washing with PBS, the sections were blocked in 5% normal goat serum at room temperature for 1 h. Sections were then incubated with primary antibodies against COL2A1 (1:200, Abcam, Cambridge, MA, USA, ab34712), aggrecan (1:200, Proteintech, Rosemont, IL, USA; 13880-1-AP) and MMP13 (1:200, Proteintech, 18165-1-AP) overnight at 4 °C. .. Next day the slides were incubated with secondary biotinylated goat anti-rabbit antibody (1:300, Jackson ImmunoResearch Laboratories, West Grove, PA, USA) for one hour at room temperature followed by chromogen DAB staining (ZSGB-BIO Co., Ltd., Beijing, China, cat ZLI-9018) and hematoxylin counterstaining. ..

    Article Title: The role of pituitary adenylate cyclase-activating polypeptide neurons in the hypothalamic ventromedial nucleus and the cognate PAC1 receptor in the regulation of hedonic feeding
    Article Snippet: Sections were then washed with 0.1 M sodium phosphate buffer (PBS; pH 7.4), and then processed overnight with a polyclonal antibody directed against PAC1R (ABCAM; Cambridge, MA, United States; AB28670; 1:500 dilution). .. The following day, two 15-min washes with PBS, and a 2 h incubation period with secondary biotinylated goat anti-rabbit antibody (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, United States; 1:300), then three 15 min washes with PBS and another 2 h overlay with streptavidin-Alexa Fluor 546 (Molecular Probes Inc., Eugene, OR, PA, United States 1:600) was conducted. ..

    Saline:

    Article Title: Vascular Endothelial Growth Factor a Promotes Chronic Itch via VEGFA-VEGFR2-PI3K-TRPV1 Axis in Allergic Contact Dermatitis
    Article Snippet: The primary antibody for VEGFA (1:1000 dilution, rabbit; Proteintech) was left overnight at 4°C. .. After washing sections thrice with phosphate-buffered saline solution (PBS), a biotinylated goat anti-rabbit antibody (1:1000, Jackson ImmunoResearch) was added for 30 minutes. .. Finally, the skin was visualized with 3,3′-diaminobenzidine (DAB, Absin), counterstained with hematoxylin (Servicebio), dehydrated, and mounted.

    Article Title: Vascular Endothelial Growth Factor a Promotes Chronic Itch via VEGFA-VEGFR2-PI3K-TRPV1 Axis in Allergic Contact Dermatitis
    Article Snippet: The primary antibody for VEGFA (1:1000 dilution, rabbit; Proteintech) was left overnight at 4°C. .. After washing sections thrice with phosphate-buffered saline solution (PBS), a biotinylated goat anti-rabbit antibody (1:1000, Jackson ImmunoResearch) was added for 30 minutes. .. Finally, the skin was visualized with 3,3′-diaminobenzidine (DAB, Absin), counterstained with hematoxylin (Servicebio), dehydrated, and mounted.

    Immunohistochemical staining:

    Article Title: The clinical-stage drug BTZ-043 accumulates in murine tuberculosis lesions and efficiently acts against Mycobacterium tuberculosis.
    Article Snippet: Cryosectionswerefixedwith 4%paraformaldehyde/PBS (24 h or 1 h for non-irradiated or irradiated sections, respectively) prior to staining with haematoxylin and eosin (HE), ZN or Heidenhain’s azan trichrome. .. For immunohistochemical analysis ZN stained cryosections were probed with rabbit polyclonal CD68 antibody (1:200, ab125212, Abcam, Cambridge, UK) which was detected with biotinylated goat anti-rabbit antibody (1:100, 111-065-144, Jackson Immunoresearch, Suffolk, UK)13. .. To visualize lipid droplets, irradiated cryosections were fixed (10% ice-cold formalin/PBS), washed with 60% 2-propanol, and incubated in Oil Red O solution (O0625, Sigma-Aldrich, Taufkirchen, Germany).

    Article Title: The clinical-stage drug BTZ-043 accumulates in murine tuberculosis lesions and efficiently acts against Mycobacterium tuberculosis
    Article Snippet: Cryosections were fixed with 4% paraformaldehyde/PBS (24 h or 1 h for non-irradiated or irradiated sections, respectively) prior to staining with haematoxylin and eosin (HE), ZN or Heidenhain’s azan trichrome. .. For immunohistochemical analysis ZN stained cryosections were probed with rabbit polyclonal CD68 antibody (1:200, ab125212, Abcam, Cambridge, UK) which was detected with biotinylated goat anti-rabbit antibody (1:100, 111-065-144, Jackson Immunoresearch, Suffolk, UK) . .. To visualize lipid droplets, irradiated cryosections were fixed (10% ice-cold formalin/PBS), washed with 60% 2-propanol, and incubated in Oil Red O solution (O0625, Sigma-Aldrich, Taufkirchen, Germany).

    Staining:

    Article Title: The clinical-stage drug BTZ-043 accumulates in murine tuberculosis lesions and efficiently acts against Mycobacterium tuberculosis.
    Article Snippet: Cryosectionswerefixedwith 4%paraformaldehyde/PBS (24 h or 1 h for non-irradiated or irradiated sections, respectively) prior to staining with haematoxylin and eosin (HE), ZN or Heidenhain’s azan trichrome. .. For immunohistochemical analysis ZN stained cryosections were probed with rabbit polyclonal CD68 antibody (1:200, ab125212, Abcam, Cambridge, UK) which was detected with biotinylated goat anti-rabbit antibody (1:100, 111-065-144, Jackson Immunoresearch, Suffolk, UK)13. .. To visualize lipid droplets, irradiated cryosections were fixed (10% ice-cold formalin/PBS), washed with 60% 2-propanol, and incubated in Oil Red O solution (O0625, Sigma-Aldrich, Taufkirchen, Germany).

    Article Title: The clinical-stage drug BTZ-043 accumulates in murine tuberculosis lesions and efficiently acts against Mycobacterium tuberculosis
    Article Snippet: Cryosections were fixed with 4% paraformaldehyde/PBS (24 h or 1 h for non-irradiated or irradiated sections, respectively) prior to staining with haematoxylin and eosin (HE), ZN or Heidenhain’s azan trichrome. .. For immunohistochemical analysis ZN stained cryosections were probed with rabbit polyclonal CD68 antibody (1:200, ab125212, Abcam, Cambridge, UK) which was detected with biotinylated goat anti-rabbit antibody (1:100, 111-065-144, Jackson Immunoresearch, Suffolk, UK) . .. To visualize lipid droplets, irradiated cryosections were fixed (10% ice-cold formalin/PBS), washed with 60% 2-propanol, and incubated in Oil Red O solution (O0625, Sigma-Aldrich, Taufkirchen, Germany).

    Article Title: Tree shrew as a new animal model for musculoskeletal disorders and aging
    Article Snippet: Next, after washing with PBS, the sections were blocked in 5% normal goat serum at room temperature for 1 h. Sections were then incubated with primary antibodies against COL2A1 (1:200, Abcam, Cambridge, MA, USA, ab34712), aggrecan (1:200, Proteintech, Rosemont, IL, USA; 13880-1-AP) and MMP13 (1:200, Proteintech, 18165-1-AP) overnight at 4 °C. .. Next day the slides were incubated with secondary biotinylated goat anti-rabbit antibody (1:300, Jackson ImmunoResearch Laboratories, West Grove, PA, USA) for one hour at room temperature followed by chromogen DAB staining (ZSGB-BIO Co., Ltd., Beijing, China, cat ZLI-9018) and hematoxylin counterstaining. ..



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